In cellulo structure determination of a novel cypovirus polyhedrin.

ABSTRACT: This work demonstrates that with the use of a microfocus synchrotron beam the structure of a novel viral polyhedrin could be successfully determined from microcrystals within cells, removing the preparatory step of sample isolation and maintaining a favourable biological environment. The data obtained are of high quality, comparable to that obtained from isolated crystals, and enabled a facile structure determination. A small but significant difference is observed between the unit-cell parameters and the mosaic spread of in cellulo and isolated crystals, suggesting that even these robust crystals are adversely affected by removal from the cell.

SUBMITTER: Axford D 

PROVIDER: S-EPMC4014125 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature