Validation of potential reference genes for qPCR in maize across abiotic stresses, hormone treatments, and tissue types.

ABSTRACT: The reverse transcription quantitative polymerase chain reaction (RT-qPCR) is a powerful and widely used technique for the measurement of gene expression. Reference genes, which serve as endogenous controls ensure that the results are accurate and reproducible, are vital for data normalization. To bolster the literature on reference gene selection in maize, ten candidate reference genes, including eight traditionally used internal control genes and two potential candidate genes from our microarray datasets, were evaluated for expression level in maize across abiotic stresses (cold, heat, salinity, and PEG), phytohormone treatments (abscisic acid, salicylic acid, jasmonic acid, ethylene, and gibberellins), and different tissue types. Three analytical software packages, geNorm, NormFinder, a

SUBMITTER: Lin Y 

PROVIDER: S-EPMC4014480 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature