Optical cell separation from three-dimensional environment in photodegradable hydrogels for pure culture techniques.

ABSTRACT: Cell sorting is an essential and efficient experimental tool for the isolation and characterization of target cells. A three-dimensional environment is crucial in determining cell behavior and cell fate in biological analysis. Herein, we have applied photodegradable hydrogels to optical cell separation from a 3D environment using a computer-controlled light irradiation system. The hydrogel is composed of photocleavable tetra-arm polyethylene glycol and gelatin, which optimized cytocompatibility to adjust a composition of crosslinker and gelatin. Local light irradiation could degrade the hydrogel corresponding to the micropattern image designed on a laptop; minimum resolution of photodegradation was estimated at 20 µm. Light irradiation separated an encapsulated fluorescent microbead withou

SUBMITTER: Tamura M 

PROVIDER: S-EPMC4014620 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature