Changes in gene expression in the larval gut of Ostrinia nubilalis in Response to Bacillus thuringiensis Cry1Ab protoxin ingestion.

ABSTRACT: We developed a microarray based on 2895 unique transcripts assembled from 15,000 cDNA sequences from the European corn borer (Ostrinia nubilalis) larval gut. This microarray was used to monitor gene expression in early third-instar larvae of Bacillus thuringiensis (Bt)-susceptible O. nubilalis after 6 h feeding on diet, with or without the Bt Cry1Ab protoxin. We identified 174 transcripts, for which the expression was changed more than two-fold in the gut of the larvae fed Cry1Ab protoxin (p < 0.05), representing 80 down-regulated and 94 up-regulated transcripts. Among 174 differentially expressed transcripts, 13 transcripts putatively encode proteins that are potentially involved in Bt toxicity, and these transcripts include eight serine proteases, three aminopeptidases, one alkaline phos

SUBMITTER: Yao J 

PROVIDER: S-EPMC4014733 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature