Comparison of incretin immunoassays with or without plasma extraction: Incretin secretion in Japanese patients with type 2 diabetes.

ABSTRACT:

Unlabelled

Aims/Introduction:  The effectiveness of incretin-based therapies in Asian type 2 diabetes requires investigation of the secretion and metabolism of glucose-dependent insulinotropic polypepide (GIP) and glucagon-like peptide 1 (GLP-1). Plasma extractions have been suggested to reduce variability in intact GLP-1 levels among individuals by removing interference that affects immunoassays, although no direct demonstration of this method has been reported. We have evaluated the effects of ethanol and solid-phase extractions on incretin immunoassays. We determined incretin levels during meal tolerance tests in Japanese patients with type 2 diabetes and characterized predictors for incretin secretion.

Materials and methods

  Japanese patients with type 2 diabetes (23 an

SUBMITTER: Yabe D 

PROVIDER: S-EPMC4014935 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature