Correlates between host and viral transcriptional program associated with different oncolytic vaccinia virus isolates.

ABSTRACT: Vaccinia virus (VACV) has emerged as an attractive tool in oncolytic virotherapy. VACV replication efficiency plays a crucial role in the therapeutic outcome. However, little is known about the influence of host factors on viral replication efficiency and permissiveness of a host cell line to infection and oncolysis. In this study, replication of the attenuated VACV GLV-1h68 strain and three wild-type VACV isolates was determined in two autologous human melanoma cell lines (888-MEL and 1936-MEL). Host gene expression and viral gene expression in infected cells were evaluated via respective expression array platforms. Microarray analyses followed by sequential statistical approaches characterized human genes that change specifically due to virus infection. Viral gene transcription correlate

SUBMITTER: Reinboth J 

PROVIDER: S-EPMC4015245 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature