PI3K pathway activation in high-grade ductal carcinoma in situ--implications for progression to invasive breast carcinoma.

ABSTRACT:

Purpose

To assess the prevalence of phosphoinositide 3-kinase (PI3K) pathway alterations in pure high-grade ductal carcinoma in situ (DCIS) and DCIS associated with invasive breast cancer (IBC), and to determine whether DCIS and adjacent IBCs harbor distinct PI3K pathway aberrations.

Experimental design

Eighty-nine cases of pure high-grade DCIS and 119 cases of high-grade DCIS associated with IBC were characterized according to estrogen receptor (ER) and HER2 status, subjected to immunohistochemical analysis of PTEN, INPP4B, phosphorylated (p)AKT and pS6 expression, and to microdissection followed by Sequenom genotyping of PIK3CA and AKT1 hotspot mutations.

Results

Alterations affecting the PI3K pathway were found in a subset of pure DCIS and DCIS adjacent to IBC. A s

SUBMITTER: Sakr RA 

PROVIDER: S-EPMC4015460 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature