Bufalin attenuates the stage and metastatic potential of hepatocellular carcinoma in nude mice.

ABSTRACT:

Background

Advanced hepatocellular carcinoma (HCC) patients undergo significant tumor growth and metastasis. Here, we investigated bufalin for treating HCC, which exhibits anti-tumor activities in many tumor cell lines.

Method

In our experiment, HCCLM3-R cells were injected into nude mice to form subcutaneous human HCC tumors that were implanted into the liver to establish orthotopic transplantation tumor models. Bufalin was injected intraperitoneally at 1 or 1.5 mg/kg. LY294002 (100 mg/kg), a potent inhibitor of Akt which reduced the levels of pAkt in HCCLM3 cell lines, was injected intraperitoneally into one group thrice weekly. The control was injected with an equal volume of saline. Morphological alterations were evaluated in the liver and lung by stereomicroscopy, the a

SUBMITTER: Zhang ZJ 

PROVIDER: S-EPMC4015709 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature