A novel platform for heterologous gene expression in Trichoderma reesei (Teleomorph Hypocrea jecorina).

ABSTRACT:

Background

The industrially applied filamentous fungus Trichoderma reesei has received substantial interest due to its highly efficient synthesis apparatus of cellulytic enzymes. However, the production of heterologous enzymes in T. reesei still remains low mainly due to lack of tools for genetic engineering.

Results

In this study we present new genetic tools for T. reesei to further expand its use in industrial production. We have developed an expression platform where genes are inserted into a versatile expression vector via highly efficient uracil-excision cloning and subsequently inserted into a defined position in the T. reesei genome ensuring that enzyme production from different transformants can be directly compared. The ade2 locus was selected as integration site si

SUBMITTER: Jorgensen MS 

PROVIDER: S-EPMC4015775 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature