Pten regulates homeostasis and inflammation-induced migration of myelocytes in zebrafish.

ABSTRACT:

Background

Loss of the tumor suppressor phosphatase and tensin homolog (PTEN) is frequently observed in hematopoietic malignancies. Although PTEN has been implicated in maintaining the quiescence of hematopoietic stem cells (HSCs), its role in hematopoiesis during ontogeny remains largely unexplored.

Methods

The expression of hematopoietic marker genes was analyzed via whole mount in situ hybridization assay in ptena and ptenb double mutant zebrafish. The embryonic myelopoiesis was characterized by living imaging and whole mount in situ immunofluorescence with confocal microscopy, as well as cell-specific chemical staining for neutrophils and macrophages. Analyses of the involved signaling pathway were carried out by inhibitor treatment and mRNA injection.

Results

Pte

SUBMITTER: Dong ZW 

PROVIDER: S-EPMC4015859 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature