Optimised laser microdissection of the human ocular surface epithelial regions for microarray studies.

ABSTRACT:

Background

The most important challenge of performing insitu transcriptional profiling of the human ocular surface epithelial regions is obtaining samples in sufficient amounts, without contamination from adjacent tissue, as the region of interest is microscopic and closely apposed to other tissues regions. We have effectively collected ocular surface (OS) epithelial tissue samples from the Limbal Epithelial Crypt (LEC), limbus, cornea and conjunctiva of post-mortem cadaver eyes with laser microdissection (LMD) technique for gene expression studies with spotted oligonucleotide microarrays and Gene 1.0 ST arrays.

Methods

Human donor eyes (4 pairs for spotted oligonucleotide microarrays, 3 pairs for Gene 1.0 ST arrays) consented for research were included in this study with du

SUBMITTER: Kulkarni BB 

PROVIDER: S-EPMC4015997 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature