The presence of heat-labile factors interfering with binding analysis of fibrinogen with ferritin in horse plasma.

ABSTRACT:

Background

Horse fibrinogen has been identified as a plasma specific ferritin-binding protein. There are two ways in the binding of ferritin-binding protein with ferritin: one is direct binding and the other is indirect binding which is heme-mediated. The aim of this study was to analyze the binding between horse fibrinogen and ferritin.

Findings

Although fibrinogen in horse plasma did not show the binding to ferritin coated on the plate wells, after following heat-treatment (60°C, 30 min) of horse plasma, plasma fibrinogen as well as purified horse fibrinogen bound to plates coated with horse spleen ferritin, but not with its apoferritin which lost heme as well as iron after the treatment of reducing reagent. Binding of purified or plasma fibrinogen to ferritin was inhibite

SUBMITTER: Takahashi K 

PROVIDER: S-EPMC4016576 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature