Making ends meet: chemically mediated circularization of recombinant proteins.

ABSTRACT: A selective N→S acyl transfer reaction facilitates semi-synthesis of the plant cyclotide kalata B1 from a linear precursor peptide of bacterial origin, through simple appendage of N-terminal cysteine and a thiol-labile C-terminal Gly-Cys motif. This constitutes the first synthesis of a ribosomally derived circular miniprotein, without recourse to protein splicing elements.

SUBMITTER: Cowper B 

PROVIDER: S-EPMC4016753 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature