Downregulation of β-actin and its regulatory gene HuR affect cell migration of human corneal fibroblasts.

ABSTRACT:

Purpose

In an earlier study, we showed that human antigen R (HuR) and β-actin expression levels were downregulated in fibroblasts isolated from human keratoconus stroma compared to normal corneal stroma. To further extend the finding, we determined whether HuR expression affects β-actin gene expression and in turn affects corneal fibroblast migration and wound healing.

Methods

Stromal keratocytes from normal human corneas were cultured in the presence of serum. Cells were transfected with siRNA specific for β-actin or HuR. SiRNAs specific for GAPDH or a scrambled sequence were used as positive and negative controls (siCTR) for transfection, respectively. The effects of gene silencing were analyzed at the transcriptional and translational levels. Specific proteins were immuno

SUBMITTER: Joseph R 

PROVIDER: S-EPMC4016806 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature