Neutral lipid stores and lipase PNPLA5 contribute to autophagosome biogenesis.

ABSTRACT:

Background

Autophagy is a fundamental cell biological process whereby eukaryotic cells form membranes in the cytoplasm to sequester diverse intracellular targets. Although significant progress has been made in understanding the origins of autophagosomal organelles, the source of lipids that support autophagic membrane formation remain an important open question.

Results

Here we show that lipid droplets as cellular stores of neutral lipids including triglycerides contribute to autophagic initiation. Lipid droplets, as previously shown, were consumed upon induction of autophagy by starvation. However, inhibition of autophagic maturation by blocking acidification or using dominant negative Atg4(C74A) that prohibits autophagosomal closure did not prevent disappearance of lipid d

SUBMITTER: Dupont N 

PROVIDER: S-EPMC4016984 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature