Different frequency of epidermal growth factor rs76189946 polymorphism genotype in an Iranian colorectal cancer.

ABSTRACT:

Aim

This study aimed to determinant association between rs76189946 polymorphism of EGF gene and risk of colorectal cancer in an Iranian population.

Background

Colorectal cancer (CRC) is the third most prevalent cancer in both genders worldwide. The determination of genetic variation becomes a new way to etiology of colorectal cancer. Epidermal growth factor (EGF) is a mitogen that plays an important role in cell growth and tumourigenesis, this protein acts by binding its receptor, EGFR.

Patients and methods

DNA samples taken from totally 125 CRC patients and healthy controls were amplified by polymerase chain reaction (PCR) for the rs76189946 polymorphism. Genotypes were analyzed using restriction fragment length polymorphism (RFLP). Finally to confirm the RFLP proced

SUBMITTER: Chaleshi V 

PROVIDER: S-EPMC4017547 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature