Localized drug application and sub-second voltammetric dopamine release measurements in a brain slice perfusion device.

ABSTRACT: The use of fast scan cyclic voltammetry (FSCV) to measure the release and uptake of dopamine (DA) as well as other biogenic molecules in viable brain tissue slices has gained popularity over the last 2 decades. Brain slices have the advantage of maintaining the functional three-dimensional architecture of the neuronal network while also allowing researchers to obtain multiple sets of measurements from a single animal. In this work, we describe a simple, easy-to-fabricate perfusion device designed to focally deliver pharmacological agents to brain slices. The device incorporates a microfluidic channel that runs under the perfusion bath and a microcapillary that supplies fluid from this channel up to the slice. We measured electrically evoked DA release in brain slices before and after the a

SUBMITTER: Sun M 

PROVIDER: S-EPMC4018083 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature