Simultaneous measurement of individual mitochondrial membrane potential and electrophoretic mobility by capillary electrophoresis.

ABSTRACT: Mitochondrial membrane potential varies, depending on energy demand, subcellular location, and morphology and is commonly used as an indicator of mitochondrial functional status. Electrophoretic mobility is a heterogeneous surface property reflective of mitochondrial surface composition and morphology, which could be used as a basis for separation of mitochondrial subpopulations. Since these properties are heterogeneous, methods for their characterization in individual mitochondria are needed to better design and understand electrophoretic separations of subpopulations of mitochondria. Here we report on the first method for simultaneous determination of individual mitochondrial membrane potential and electrophoretic mobility by capillary electrophoresis with laser-induced fluorescence dete

SUBMITTER: Wolken GG 

PROVIDER: S-EPMC4018156 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature