Hapten-specific naive B cells are biomarkers of vaccine efficacy against drugs of abuse.

ABSTRACT: Vaccination against drugs of abuse shows efficacy in animal models, yet few subjects achieve effective serum antibody titers in clinical studies. A barrier to translation is the lack of pre-vaccination screening assays that predict the most effective conjugate vaccines or subjects amenable to vaccination. To address this obstacle, we developed a fluorescent antigen-based enrichment method paired with flow cytometry to characterize hapten-specific B cells. Using this approach, we studied naïve and activated B cells specific for structurally-related model haptens based on derivatization of the morphinan structure at the C6 position on oxycodone or at the C8 position on hydrocodone, and showing different pre-clinical efficacy against the prescription opioid oxycodone. Prior to vaccination, na

SUBMITTER: Taylor JJ 

PROVIDER: S-EPMC4018303 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature