Antibody-dependent infection of human macrophages by severe acute respiratory syndrome coronavirus.

ABSTRACT:

Background

Public health risks associated to infection by human coronaviruses remain considerable and vaccination is a key option for preventing the resurgence of severe acute respiratory syndrome coronavirus (SARS-CoV). We have previously reported that antibodies elicited by a SARS-CoV vaccine candidate based on recombinant, full-length SARS-CoV Spike-protein trimers, trigger infection of immune cell lines. These observations prompted us to investigate the molecular mechanisms and responses to antibody-mediated infection in human macrophages.

Methods

We have used primary human immune cells to evaluate their susceptibility to infection by SARS-CoV in the presence of anti-Spike antibodies. Fluorescence microscopy and real-time quantitative reverse transcriptase polymerase cha

SUBMITTER: Yip MS 

PROVIDER: S-EPMC4018502 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature