Cellulose digestion and metabolism induced biocatalytic transitions in anaerobic microbial ecosystems.

ABSTRACT: Anaerobic digestion of highly polymerized biomass by microbial communities present in diverse microbial ecosystems is an indispensable metabolic process for biogeochemical cycling in nature and for industrial activities required to maintain a sustainable society. Therefore, the evaluation of the complicated microbial metabolomics presents a significant challenge. We here describe a comprehensive strategy for characterizing the degradation of highly crystallized bacterial cellulose (BC) that is accompanied by metabolite production for identifying the responsible biocatalysts, including microorganisms and their metabolic functions. To this end, we employed two-dimensional solid- and one-dimensional solution-state nuclear magnetic resonance (NMR) profiling combined with a metagenomic approach

SUBMITTER: Yamazawa A 

PROVIDER: S-EPMC4018678 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature