Interleukin 10 overexpression alters survival in the setting of gram-negative pneumonia following lung contusion.

ABSTRACT:

Objective

Lung contusion injury produces a vulnerable window within the inflammatory defenses of the lung that predisposes the patient to pneumonia. Interleukin 10 (IL-10) is a known anti-inflammatory mediator produced by macrophages and capable of downregulating acute lung inflammation. We investigated the impact of increased levels of IL-10 within the lung on survival and the host response to trauma in the setting of lung contusion (LC) and gram-negative pneumonia.

Design

A bitransgenic, tetracycline-inducible, lung-specific human IL-10 overexpression (IL-10 OE) mouse model and single transgenic (TG-) control mice were used. Mice underwent LC injury or sham injury (sham) at time -6 h. At time 0, animals were inoculated intratracheally with 500 colony-forming units of Klebs

SUBMITTER: Dolgachev VA 

PROVIDER: S-EPMC4019007 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature