Coronavirus replicase-reporter fusions provide quantitative analysis of replication and replication complex formation.

ABSTRACT:

Unlabelled

The replication of coronaviruses occurs in association with multiple virus-induced membrane structures that evolve during the course of infection; however, the dynamics of this process remain poorly understood. Previous studies of coronavirus replication complex organization and protein interactions have utilized protein overexpression studies and immunofluorescence of fixed cells. Additionally, live-imaging studies of coronavirus replicase proteins have used fluorescent reporter molecules fused to replicase proteins, but expressed from nonnative locations, mostly late-transcribed subgenomic mRNAs, in the presence or absence of the native protein. Thus, the timing and targeting of native replicase proteins expressed in real time from native locations in the genome remain

SUBMITTER: Freeman MC 

PROVIDER: S-EPMC4019139 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature