Phenotypic screening of a targeted mutant library reveals Campylobacter jejuni defenses against oxidative stress.

ABSTRACT: During host colonization, Campylobacter jejuni is exposed to harmful reactive oxygen species (ROS) produced from the host immune system and from the gut microbiota. Consequently, identification and characterization of oxidative stress defenses are important for understanding how C. jejuni survives ROS stress during colonization of the gastrointestinal tract. Previous transcriptomic studies have defined the genes belonging to oxidant stimulons within C. jejuni. We have constructed isogenic deletion mutants of these identified genes to assess their role in oxidative stress survival. Phenotypic screening of 109 isogenic deletion mutants identified 22 genes which were either hypersensitive or hyposensitive to oxidants, demonstrating important roles for these genes in oxidant defense. The signi

SUBMITTER: Flint A 

PROVIDER: S-EPMC4019188 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature