Involvement of heparan sulfate 3-O-sulfotransferase isoform-1 in the insulin secretion pathway.

ABSTRACT:

Unlabelled

Aims/Introduction:  Heparan sulfate (HS) mediates a variety of molecular recognition events that are essential for differentiation, morphogenesis and homeostasis through various HS forms that result from differential sulfate modification. Recently, we found that HS is localized exclusively around βß-cells in islets of adult mice and is required for insulin secretion. The aim of this study was to examine the contribution of HS sulfate groups to insulin secretion.

Materials and methods

  Glucose-induced insulin secretion (GIIS) was examined in mouse pancreatic islets, the mouse pancreatic β-cell line MIN6 cells and its derivative MIN6T3 cells after removal of sulfate groups by sodium chlorate, a competitive inhibitor of glycosaminoglycan sulfation. Quantitative reve

SUBMITTER: Takahashi I 

PROVIDER: S-EPMC4019256 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature