Vascular tone and Ca(2+) signaling in murine cremaster muscle arterioles in vivo.

ABSTRACT:

Objectives

We sought to determine some of the molecular requirements for basal state "tone" of skeletal muscle arterioles in vivo, and whether asynchronous Ca(2+) waves are involved or not.

Methods

Cremaster muscles of anesthetized exMLCK and smGCaMP2 biosensor mice were exteriorized, and the fluorescent arterioles were visualized with wide-field, confocal or multiphoton microscopy to observe Ca(2+) signaling and arteriolar diameter.

Results

Basal state tone of the arterioles was ~50%. Local block of Ang-II receptors (AT1 ) or α1 -adrenoceptors (α1 -AR) had no effect on diameter, nor did complete block of sympathetic nerve activity (SNA). Inhibition of phospholipase C caused dilation nearly to the Ca(2+) -free (passive) diameter, as did exposure to nifedipine or 2-APB

SUBMITTER: Mauban JR 

PROVIDER: S-EPMC4019383 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature