Raman Labeled Nanoparticles: Characterization of Variability and Improved Method for Unmixing.

ABSTRACT: Raman spectroscopy can differentiate the spectral fingerprints of many molecules, resulting in potentially high multiplexing capabilities of Raman-tagged nanoparticles. However, accurate quantitative unmixing of Raman spectra is challenging because of potential overlaps between Raman peaks from each molecule as well as slight variations in the location, height and width of the very narrow peaks. If not accounted for properly, even minor fluctuations in the spectra may produce significant error which will ultimately result in poor unmixing accuracy. The objective of our study was to develop and validate a mathematical model of the Raman spectra of nanoparticles to unmix the contributions from each nanoparticle allowing simultaneous quantitation of several nanoparticle concentrations during

SUBMITTER: Kode K 

PROVIDER: S-EPMC4019428 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature