Cell type-specific expression of SEPT3-homology subgroup members controls the subunit number of heteromeric septin complexes.

ABSTRACT: Septins are filament-forming proteins important for organizing the cortex of animal and fungal cells. In mammals, 13 septin paralogues were recently shown to assemble into core heterohexamer and heterooctamer complexes, which serve as building blocks for apolar filamentous structures that differ among cell types. To determine how tissue-specific septin paralogue expression may shape core heteromer repertoires and thereby modulate properties of septin filaments, we devised protocols to analyze native septin heteromers with distinct numbers of subunits. Our evidence based on genetically manipulated human cells supports and extends recent concepts of homology subgroup-restricted assembly into distinct categories of apolar heterohexamers and heterooctamers. We also identify a category of tetra

SUBMITTER: Sellin ME 

PROVIDER: S-EPMC4019491 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature