Significant productivity improvement of the baculovirus expression vector system by engineering a novel expression cassette.

ABSTRACT: Here we describe the development of a baculovirus vector expression cassette containing rearranged baculovirus-derived genetic regulatory elements. This newly designed expression cassette conferred significant production improvements to the baculovirus expression vector system (BEVS), including prolonged cell integrity after infection, improved protein integrity, and around 4-fold increase in recombinant protein production yields in insect cells with respect to a standard baculovirus vector. The expression cassette consisted of a cDNA encoding for the baculovirus transactivation factors IE1 and IE0, expressed under the control of the polyhedrin promoter, and a homologous repeated transcription enhancer sequence operatively cis-linked to the p10 promoter or to chimeric promoters containing

SUBMITTER: Gomez-Sebastian S 

PROVIDER: S-EPMC4019511 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature