Derivation of transgene-free human induced pluripotent stem cells from human peripheral T cells in defined culture conditions.

ABSTRACT: Recently, induced pluripotent stem cells (iPSCs) were established as promising cell sources for revolutionary regenerative therapies. The initial culture system used for iPSC generation needed fetal calf serum in the culture medium and mouse embryonic fibroblast as a feeder layer, both of which could possibly transfer unknown exogenous antigens and pathogens into the iPSC population. Therefore, the development of culture systems designed to minimize such potential risks has become increasingly vital for future applications of iPSCs for clinical use. On another front, although donor cell types for generating iPSCs are wide-ranging, T cells have attracted attention as unique cell sources for iPSCs generation because T cell-derived iPSCs (TiPSCs) have a unique monoclonal T cell receptor genom

SUBMITTER: Kishino Y 

PROVIDER: S-EPMC4019536 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature