Comprehensive selection of reference genes for gene expression normalization in sugarcane by real time quantitative rt-PCR.

ABSTRACT: The increasingly used real time quantitative reverse transcription-PCR (qRT-PCR) method for gene expression analysis requires one or several reference gene(s) acting as normalization factor(s). In order to facilitate gene expression studies in sugarcane (Saccharum officinarum), a non-model plant with limited genome information, the stability of 13 candidate reference genes was evaluated. The geNorm, NormFinder and deltaCt methods were used for selecting stably expressed internal controls across different tissues and under various experimental treatments. These results revealed that, among these 13 candidate reference genes, GAPDH, eEF-1a and eIF-4α were the most stable and suitable for use as normalization factors across all various experimental samples. In addition, APRT could be a candid

SUBMITTER: Ling H 

PROVIDER: S-EPMC4019594 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature