PET imaging to non-invasively study immune activation leading to antitumor responses with a 4-1BB agonistic antibody.

ABSTRACT:

Background

Molecular imaging with positron emission tomography (PET) may allow the non-invasive study of the pharmacodynamic effects of agonistic monoclonal antibodies (mAb) to 4-1BB (CD137). 4-1BB is a member of the tumor necrosis factor family expressed on activated T cells and other immune cells, and activating 4-1BB antibodies are being tested for the treatment of patients with advanced cancers.

Methods

We studied the antitumor activity of 4-1BB mAb therapy using [(18) F]-labeled fluoro-2-deoxy-2-D-glucose ([(18) F]FDG) microPET scanning in a mouse model of colon cancer. Results of microPET imaging were correlated with morphological changes in tumors, draining lymph nodes as well as cell subset uptake of the metabolic PET tracer in vitro.

Results

The administratio

SUBMITTER: Escuin-Ordinas H 

PROVIDER: S-EPMC4019904 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature