Natural insertions in rice commonly form tandem duplications indicative of patch-mediated double-strand break induction and repair.

ABSTRACT: The insertion of DNA into a genome can result in the duplication and dispersal of functional sequences through the genome. In addition, a deeper understanding of insertion mechanisms will inform methods of genetic engineering and plant transformation. Exploiting structural variations in numerous rice accessions, we have inferred and analyzed intermediate length (10-1,000 bp) insertions in plants. Insertions in this size class were found to be approximately equal in frequency to deletions, and compound insertion-deletions comprised only 0.1% of all events. Our findings indicate that, as observed in humans, tandem or partially tandem duplications are the dominant form of insertion (48%), although short duplications from ectopic donors account for a sizable fraction of insertions in rice (38%

SUBMITTER: Vaughn JN 

PROVIDER: S-EPMC4020087 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature