Combination treatment of db/db mice with exendin-4 and gastrin preserves β-cell mass by stimulating β-cell growth and differentiation.

ABSTRACT:

Unlabelled

Aim/Introduction:  Preservation of β-cell mass is crucial for maintaining long-term glucose homeostasis. Therapies based on incretin and its mimetics are expected to achieve this goal through various biological functions, particularly the restoration of β-cell mass. Here we tested the effects of gastrin and exendin-4 in type 2 diabetic animals.

Materials and methods

  The effects of exendin-4 and gastrin on β-cell function and mass were examined in 8-week-old db/db mice. INS-1 beta cells and AR42J cells were used to determine the molecular mechanism underlying the effects of the two agents. Immunohistochemistry, western blotting and RT-PCR assays were used to assess the biological effects of the two agents.

Results

  Two weeks of combination administration

SUBMITTER: Tamaki M 

PROVIDER: S-EPMC4020718 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature