Apocynin attenuates ventilator-induced lung injury in an isolated and perfused rat lung model.

ABSTRACT:

Rationale

Apocynin suppresses the generation of reactive oxygen species (ROS) that are implicated in ventilator-induced lung injury (VILI). We thus hypothesized that apocynin attenuates VILI.

Methods

VILI was induced by mechanical ventilation with tidal volume (V(t)) of 15 ml/kg in isolated and perfused rat lung. Apocynin was administered in the perfusate at onset of mechanical ventilation. A group ventilated with low V(t) of 5 ml/kg served as control. Hemodynamics, lung injury indices, inflammatory responses, and activation of apoptotic pathways were determined upon completion of mechanical ventilation.

Results

There was an increase in lung permeability and lung weight gain after mechanical ventilation with high V(t), compared with low V (t). Levels of inflammatory c

SUBMITTER: Chiang CH 

PROVIDER: S-EPMC4020846 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature