Identified members of the Streptomyces lividans AdpA regulon involved in differentiation and secondary metabolism.

ABSTRACT:

Background

AdpA is a key transcriptional regulator involved in the complex growth cycle of Streptomyces. Streptomyces are Gram-positive bacteria well-known for their production of secondary metabolites and antibiotics. Most work on AdpA has been in S. griseus, and little is known about the pathways it controls in other Streptomyces spp. We recently discovered interplay between ClpP peptidases and AdpA in S. lividans. Here, we report the identification of genes directly regulated by AdpA in S. lividans.

Results

Microarray experiments revealed that the expression of hundreds of genes was affected in a S. lividans adpA mutant during early stationary phase cultures in YEME liquid medium. We studied the expression of the S. lividans AdpA-regulated genes by quantitative real-time

SUBMITTER: Guyet A 

PROVIDER: S-EPMC4021200 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature