A reduced genome decreases the host carrying capacity for foreign DNA.

ABSTRACT:

Background

Host-plasmid interactions have been discussed largely in terms of the influences of plasmids, whereas the contributions of variations in host genomes to host interactions with foreign DNA remain unclear. A strain with a so-called "clean genome" (i.e., MDS42) of reduced genome size has recently been generated from the wild-type strain MG1655, a commonly used host strain. A quantitative evaluation of the influence of plasmid burdens in these two Escherichia coli strains can not only provide an understanding of how a reduced genome responds to foreign DNA but also offer insights into the proper application of these strains.

Results

The decreases in growth caused by the cost of carrying foreign DNA were similar for the wild-type and clean-genome strains. A negative co

SUBMITTER: Akeno Y 

PROVIDER: S-EPMC4021254 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature