Expression of endoglucanases in Pichia pastoris under control of the GAP promoter.

ABSTRACT:

Background

Plant-derived biomass is a potential alternative to fossil feedstocks for a greener economy. Enzymatic saccharification of biomass has been studied extensively and endoglucanases have been found to be a prerequisite for quick initial liquefaction of biomass under industrial conditions. Pichia pastoris, widely used for heterologous protein expression, can be utilized for fungal endoglucanase production. The recently marketed PichiaPink™ expression system allows for rapid clone selection, and employs the methanol inducible AOX1 promoter to ensure high protein expression levels. However, methanol is toxic and poses a fire hazard, issues which become more significant at an industrial scale. It is possible to eliminate these risks and still maintain high productivity by switc

SUBMITTER: Varnai A 

PROVIDER: S-EPMC4021498 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature