N-nitroso-N-ethylurea activates DNA damage surveillance pathways and induces transformation in mammalian cells.

ABSTRACT:

Background

The DNA damage checkpoint signalling cascade sense damaged DNA and coordinates cell cycle arrest, DNA repair, and/or apoptosis. However, it is still not well understood how the signalling system differentiates between different kinds of DNA damage. N-nitroso-N-ethylurea (NEU), a DNA ethylating agent induces both transversions and transition mutations.

Methods

Immunoblot and comet assays were performed to detect DNA breaks and activation of the canonical checkpoint signalling kinases following NEU damage upto 2 hours. To investigate whether mismatch repair played a role in checkpoint activation, knock-down studies were performed while flow cytometry analysis was done to understand whether the activation of the checkpoint kinases was cell cycle phase specific. Final

SUBMITTER: Bodakuntla S 

PROVIDER: S-EPMC4021545 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature