Surprising base pairing and structural properties of 2'-trifluoromethylthio-modified ribonucleic acids.

ABSTRACT: The chemical synthesis of ribonucleic acids (RNA) with novel chemical modifications is largely driven by the motivation to identify eligible functional probes for the various applications in life sciences. To this end, we have a strong focus on the development of novel fluorinated RNA derivatives that are powerful in NMR spectroscopic analysis of RNA folding and RNA ligand interactions. Here, we report on the synthesis of 2'-SCF3 pyrimidine nucleoside containing oligoribonucleotides and the comprehensive investigation of their structure and base pairing properties. While this modification has a modest impact on thermodynamic stability when it resides in single-stranded regions, it was found to be destabilizing to a surprisingly high extent when located in double helical regions. Our NMR sp

SUBMITTER: Kosutic M 

PROVIDER: S-EPMC4021565 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature