A novel anti-CD19 monoclonal antibody (GBR 401) with high killing activity against B cell malignancies.

ABSTRACT:

Background

CD19 is a B cell lineage specific surface receptor whose broad expression, from pro-B cells to early plasma cells, makes it an attractive target for the immunotherapy of B cell malignancies. In this study we present the generation of a novel humanized anti-CD19 monoclonal antibody (mAb), GBR 401, and investigate its therapeutic potential on human B cell malignancies.

Methods

GBR 401 was partially defucosylated in order to enhance its cytotoxic function. We analyzed the in vitro depleting effects of GBR 401 against B cell lines and primary malignant B cells from patients in the presence or in absence of purified NK cells isolated from healthy donors. In vivo, the antibody dependent cellular cytotoxicity (ADCC) efficacy of GBR 401 was assessed in a B cell depletion

SUBMITTER: Breton CS 

PROVIDER: S-EPMC4021825 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature