Cisplatin-induced epigenetic activation of miR-34a sensitizes bladder cancer cells to chemotherapy.

ABSTRACT:

Background

Accumulating evidence suggests a tumor suppressive role for miR-34a in human carcinogenesis. However, its precise biological role remains largely elusive. This study aimed to reveal the association of the miR-34a expression and its modulation of sensitivity to cisplatin in muscle-invasive bladder cancer (MIBC).

Methods

miR-34a expression in MIBC cell lines and patient tissues was investigated using qPCR. The methylation analysis of miR-34a promoter region was performed by MassARRAY. Synthetic short single or double stranded RNA oligonucleotides and lentiviral vector were used to regulate miR-34a expression in MIBC cells to investigate its function in vitro and in vivo.

Results

miR-34a expression was frequently decreased in MIBC tissues and cell lines throug

SUBMITTER: Li H 

PROVIDER: S-EPMC4022035 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature