Phosphoproteomic analysis of the non-seed vascular plant model Selaginella moellendorffii.

ABSTRACT:

Background

Selaginella (Selaginella moellendorffii) is a lycophyte which diverged from other vascular plants approximately 410 million years ago. As the first reported non-seed vascular plant genome, Selaginella genome data allow comparative analysis of genetic changes that may be associated with land plant evolution. Proteomics investigations on this lycophyte model have not been extensively reported. Phosphorylation represents the most common post-translational modifications and it is a ubiquitous regulatory mechanism controlling the functional expression of proteins inside living organisms.

Results

In this study, polyethylene glycol fractionation and immobilized metal ion affinity chromatography were employed to isolate phosphopeptides from wild-growing Selaginella. Using

SUBMITTER: Chen X 

PROVIDER: S-EPMC4022089 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature