Cloning and characterization of glutamate receptor subunit 4 (GLUA4) and its alternatively spliced isoforms in turtle brain.

ABSTRACT: Ionotropic glutamate receptors sensitive to α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), GluAs, play an important role in neural development, synaptic plasticity, and neurodegeneration. Previous studies using an in vitro model of eyeblink classical conditioning in pond turtles suggested that acquisition of conditioning is associated with synaptic delivery of AMPA receptors containing GluA4 subunits. However, sequences of the GluA4 subunit, expression profile, and its alternatively spliced isoforms in turtle brain have not been previously determined. The sequence and domain structure of turtle GluA4 (tGluA4) and its splice variants was characterized. We found ten isoforms of tGluA4 including several previously unidentified truncated variants. Analysis of the nucleotide seque

SUBMITTER: Sabirzhanov B 

PROVIDER: S-EPMC4022150 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature