Global transcriptional analysis reveals surface remodeling of Anaplasma marginale in the tick vector.

ABSTRACT:

Background

Pathogens dependent upon vectors for transmission to new hosts undergo environment specific changes in gene transcription dependent on whether they are replicating in the vector or the mammalian host. Differential gene transcription, especially of potential vaccine candidates, is of interest in Anaplasma marginale, the tick-borne causative agent of bovine anaplasmosis.

Methods

RNA-seq technology allowed a comprehensive analysis of the transcriptional status of A. marginale genes in two conditions: bovine host blood and tick derived cell culture, a model for the tick vector. Quantitative PCR was used to assess transcription of a set of genes in A. marginale infected tick midguts and salivary glands at two time points during the transmission cycle.

Results

Ge

SUBMITTER: Hammac GK 

PROVIDER: S-EPMC4022386 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature