A convenient, optimized pipeline for isolation, fluorescence microscopy and molecular analysis of live single cells.

ABSTRACT:

Background

Heterogeneity within cell populations is relevant to the onset and progression of disease, as well as development and maintenance of homeostasis. Analysis and understanding of the roles of heterogeneity in biological systems require methods and technologies that are capable of single cell resolution. Single cell gene expression analysis by RT-qPCR is an established technique for identifying transcriptomic heterogeneity in cellular populations, but it generally requires specialized equipment or tedious manipulations for cell isolation.

Results

We describe the optimization of a simple, inexpensive and rapid pipeline which includes isolation and culture of live single cells as well as fluorescence microscopy and gene expression analysis of the same single cells by RT

SUBMITTER: Yaron JR 

PROVIDER: S-EPMC4022543 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature