Toward single-molecule optical mapping of the epigenome.

ABSTRACT: The past decade has seen an explosive growth in the utilization of single-molecule techniques for the study of complex systems. The ability to resolve phenomena otherwise masked by ensemble averaging has made these approaches especially attractive for the study of biological systems, where stochastic events lead to inherent inhomogeneity at the population level. The complex composition of the genome has made it an ideal system to study at the single-molecule level, and methods aimed at resolving genetic information from long, individual, genomic DNA molecules have been in use for the last 30 years. These methods, and particularly optical-based mapping of DNA, have been instrumental in highlighting genomic variation and contributed significantly to the assembly of many genomes including the

SUBMITTER: Levy-Sakin M 

PROVIDER: S-EPMC4022788 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature