The adaptation of a CTN-1 rabies virus strain to high-titered growth in chick embryo cells for vaccine development.

ABSTRACT:

Background

Rabies virus is the causative agent of rabies, a central nervous system disease that is almost invariably fatal. Currently vaccination is the most effective strategy for preventing rabies, and vaccines are most commonly produced from cultured cells. Although the vaccine strains employed in China include CTN, aG, PM and PV, there are no reports of strains that are adapted to primary chick embryo cells for use in human rabies prevention in China.

Results

Rabies virus strain CTN-1 V was adapted to chick embryo cells by serial passage to obtain the CTNCEC25 strain. A virus growth curve demonstrated that the CTNCEC25 strain achieved high titers in chick embryo cells and was nonpathogenic to adult mice by intracerebral inoculation. A comparison of the structural protein

SUBMITTER: Guo C 

PROVIDER: S-EPMC4023167 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature