Genome-wide variations in a natural isolate of the nematode Caenorhabditis elegans.

ABSTRACT:

Background

Increasing genetic and phenotypic differences found among natural isolates of C. elegans have encouraged researchers to explore the natural variation of this nematode species.

Results

Here we report on the identification of genomic differences between the reference strain N2 and the Hawaiian strain CB4856, one of the most genetically distant strains from N2. To identify both small- and large-scale genomic variations (GVs), we have sequenced the CB4856 genome using both Roche 454 (~400 bps single reads) and Illumina GA DNA sequencing methods (101 bps paired-end reads). Compared to previously described variants (available in WormBase), our effort uncovered twice as many single nucleotide variants (SNVs) and increased the number of small InDels almost 20-fold. Moreov

SUBMITTER: Vergara IA 

PROVIDER: S-EPMC4023591 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature