Patterns of homoeologous gene expression shown by RNA sequencing in hexaploid bread wheat.

ABSTRACT:

Background

Bread wheat (Triticum aestivum) has a large, complex and hexaploid genome consisting of A, B and D homoeologous chromosome sets. Therefore each wheat gene potentially exists as a trio of A, B and D homoeoloci, each of which may contribute differentially to wheat phenotypes. We describe a novel approach combining wheat cytogenetic resources (chromosome substitution 'nullisomic-tetrasomic' lines) with next generation deep sequencing of gene transcripts (RNA-Seq), to directly and accurately identify homoeologue-specific single nucleotide variants and quantify the relative contribution of individual homoeoloci to gene expression.

Results

We discover, based on a sample comprising ~5-10% of the total wheat gene content, that at least 45% of wheat genes are expressed fro

SUBMITTER: Leach LJ 

PROVIDER: S-EPMC4023595 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature